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M9480847.TXT
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1994-09-05
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Document 0847
DOCN M9480847
TI Peripheral blood and milk-derived leukocytes harbor BIV provirus.
DT 9410
AU Nash JW; Hanson LA; St. Cyr Coats K; Mississippi State University.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:486 (abstract no. T-22). Unique
Identifier : AIDSLINE ASM94/94313098
AB Bovine immunodeficiency virus (BIV), a lentivirus, infects a large
population of cattle in the southern United States. The mode of
transmission of BIV is unknown, but its similarity to HIV suggests
analogous mechanisms of transmission. The purpose of this study was to
develop a PCR probe to detect BIV proviral sequences in cells which are
likely vehicles for BIV transmission. DNA was prepared from peripheral
blood (PBL) and milk-derived leukocytes and used as template for PCR.
The proviral target for PCR amplification was a 234 bp fragment within a
highly conserved region of the BIV pol gene. DNA fragments of
approximately 234 bp were amplified from the DNA of both PBL and
milk-derived leukocytes from BIV seropositive animals. Nucleotide
sequencing of the amplified products confirmed their BIV specificity.
Further study is necessary to determine the role of whole blood and milk
in transmission of BIV in natural infections.
DE Animal Base Sequence Cattle Cattle Diseases Comparative Study
Conserved Sequence DNA, Viral/*ANALYSIS/BLOOD Female Genes, pol
Immunodeficiency Virus, Bovine/GENETICS/*ISOLATION & PURIF Lentivirus
Infections/DIAGNOSIS/VETERINARY Leukocytes/*MICROBIOLOGY
Milk/*MICROBIOLOGY Polymerase Chain Reaction/METHODS
Proviruses/GENETICS/ISOLATION & PURIF MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).